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RESEARCH ARTICLE
Year : 2013  |  Volume : 2  |  Issue : 2  |  Page : 171-178

Design of intelligent polymer of gelatin- poly N-isopropyla-crylamide under gamma radiation for cellular applications


1 Proteomics Research Center, Shahid Beheshti University of Medical sciences; Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
3 Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
4 Medical Education Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5 Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

Correspondence Address:
Saeed Heidari-Keshel
Proteomics Research Center, Shahid Beheshti University of Medical sciences; Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran
Iran
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Source of Support: None, Conflict of Interest: None


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Thermo-sensitive polymers were prepared by graft copolymerization of gelatin with N-Isopropylacrylamide via gamma radiation. Characterization of polymers such as DSC analysis, swelling in different ratios and cell assays were investigated. DSC and solubility analysis showed gelatin and N-Isopropylacrylamide monomers were grafted via gamma radiation successfully. Results show swelling of samples increased as gelatin increased. Swelling ratio and curves results administrated hyd-ro-philicity / hydrophobicity of hydrogel that this property is due to presence of N-Isopropylacrylamide in different temperatures. The polymer was tested for harvesting epithelial cells after carrying out cell culture at 37 °C and incubating the confluent cells at 10°C for spontaneous detachment of cell sheet from polymer surface without enzyme treatment. These unique properties of the hydrogel would make it a promising support for drug delivery systems and tissue regeneration.


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