• Users Online: 363
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 

 Table of Contents  
Year : 2020  |  Volume : 9  |  Issue : 2  |  Page : 189-195

Synthesis and evaluation of thiadiazole-based antileishmanial agents

1 Department of Medicinal Chemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran
2 Department of Mycology and Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran

Date of Submission09-Feb-2020
Date of Acceptance10-May-2020
Date of Web Publication07-Oct-2020

Correspondence Address:
Dr. Elham Jafari
Department of Medicinal Chemistry and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan.
I.R. Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jrptps.JRPTPS_3_20

Rights and Permissions

Background and Objectives: The 1, 3, 4-thiadiazole scaffold is one of the principal structural components, in a variety of drug categories such as antimicrobial, anti-inflammatory, antineoplastic, and antileishmanial agents. Considering the reported antileishmanial effects of thiadiazole derivatives and the importance of this disease, some of the thiadiazole derivatives with modifications at sulfur atom or amine group attached to the 2-position were synthesized and evaluated for antileishmanial activity. Materials and Methods: Derivatives of 1,3,4-thiadiazole including 2-substituted-thio-1,3,4-thiadiazoles bearing (5-(4-nitrobenzylideneamino) or 5-amino (II, IV, V) and one derivative of 2-substituted-amino-1,3,4-thiadiazole bearing (5- (4-nitrophenyl) (VII) were synthesized and evaluated for their in vitro antileishmanial activity against promastigote and amastigote forms of the Leishmania major. Results: The most active compound was found to be compound II after 24-h incubation against promastigotes and amastigotes with the half maximal inhibitory concentration (IC50) values of 44.4 µM and 64.7 µM, respectively. Conclusion: All of the synthesized compounds showed good antileishmanial activity against both forms of L. major after 48 and 72h incubation.

Keywords: Amastigote, antileishmanial activity, leishmania major, promastigote, thiadiazole

How to cite this article:
Hassanzadeh F, Jafari E, Saeedi M, Saberi S. Synthesis and evaluation of thiadiazole-based antileishmanial agents. J Rep Pharma Sci 2020;9:189-95

How to cite this URL:
Hassanzadeh F, Jafari E, Saeedi M, Saberi S. Synthesis and evaluation of thiadiazole-based antileishmanial agents. J Rep Pharma Sci [serial online] 2020 [cited 2023 Sep 21];9:189-95. Available from: https://www.jrpsjournal.com/text.asp?2020/9/2/189/297350

  Introduction Top

Leishmaniasis is a disease induced by the parasites of the genus Leishmania.[1],[2],[3],[4]Leishmania parasites have two stage life cycles including: extracellular and an intracellular.[5],[6] Antileishmanial effects are related with disparate heterocyclic core such as piperazine, pyrimidine, azoles (imidazole, 1, 2, 4 triazole, isoxazole, pyrazole, and thiadiazole), quinazoline, and indole[7] [Figure 1]. Thiadiazole has a constrained pharmacophore with two electron donor systems, which can act as “hydrogen binding domain.”[8],[9] The sulfur atom of thiadiazole increases lipophilicity resulting in good cell permeability and bioavailability.[10],[11] This pharmacologically significant scaffold has shown several biological activities including anticancer, antibacterial,[9],[10],[11],[12],[13],[14] anticonvulsant, antitubercular, anti-inflammatory, and antileishmanial effects.[9],[13] Literature surveys have shown antileishmanial activity for 1, 3, 4-thiadiazoles; however, linkage to other heterocycles can mutate the bioactivity, contingent on the type, and the position of substituent.[1],[6],[15],[16],[17] One of the proposed mechanisms for antileishmanial effects of thiadiazole derivatives is affinity for attaching to the sulphydryl groups of parasit enzymes or proteins.[10],[18] It has been reported that 2-mino-1, 3, 4 -thiadiazole derivatives represented antileishmanial properties through inhibition of parasitic enzymes.[10],[18] Antileishmanial activity was reported for derivatives of ethyl (5-amino-1, 3, 4-thiadiazoe-2-yl) (hexahydropyrimidine or imidazolidin-2-ylidene) acetate by Ram et al.[7],[19] The use of nitro heterocyclic structures such as 5-nitrofurans, 5-nitrothiophenes, and 5-nitroimidazoles in the expansion of antiparasitic agents has been determined.[15],[16],[17] The structure–activity relationship (SAR) study showed that the S atom joined to the 2-position of the thiadiazole ring has a high pliability for structural rectification to retain good antileishmanial activity.[16]
Figure 1: Different heterocyclic nucleus with antileishmanial activity

Click here to view

According to the incidence of leishmanial infection and important problems including the great cost of drugs, emergence of immunosuppressive illnesses such as AIDS and drug resistance,[1] in this research a series of thiadiazole derivatives with modifications at S-pendant or amino group joined to the 2-position were synthesized and evaluated for antileishmanial activity.

  Materials and Methods Top


All reagents and solvents were obtained from commercial suppliers such as Merck (Germany) and Aldrich (USA). Merck silica gel 60 F254 plates (Germany) were used for analytical thin-layer chromatography (TLC) and monitoring of reactions. Proton nuclear magnetic resonance (HNMR) spectra were registered using a (Bruker 400 MHz, Germany) spectrometer. Infrared (IR) (potassium bromide discs) was recorded with a WQF-510 FT-IR spectrophotometer (China). Melting points were determined using electrothermal 9200 melting point instrument (UK) and are uncorrected. Mass spectra were registered on Agilent Technologies 5975C mass spectrometer (USA).


Synthesis of 2-(5-amino-1, 3, 4-thiadiazol)-1-(4-bromophenyl) ethanone (I)

To a mixture of 5-amino-1, 3, 4-thiadiazole-2-thiol (3 mmole) and potassium hydroxide (3 mmol) in ethanol, 2-bromo-1 (4-bromophenyl) ethanone (3 mmol) was added. Then, the reaction mixture was allowed to stir overnight. Obtained white solid was filtered and washed with water and crystallized from ethanol to give compound I[16][Scheme 1].
Scheme 1: Synthetic route to compounds (I–V). KOH, BrCH2COPhBr, EtOH; 4-NO2Ph-CHO, H2SO4, EtOH, 6h reflux; ClCH2COOEt,KOH, EtOH; NH2NH2, EtOH, 3h reflux; 4-NO2Ph-CHO, MeOH, Glacial CH3COOH, 2h reflux

Click here to view

Synthesis of 2-(5-(4-nitrobenzylideneamino)-1, 3, 4-thiadiazol-2-ylthio)-1-(4-bromophenyl) ethanone (II)

Equimolar quantities of compound I (1.5 mmol), 4-nitro-benzaldehyde, and catalytic amount of concentrated sulfuric acid (2–5 drops) were dissolved in absolute ethanol. The reaction mixture was refluxed for 6h until the end of the reaction that determined by TLC. Then, solvent was removed under reduced pressure, ice cold water was added, and the product was extracted using chloroform. Organic layer was evaporated to dryness. Obtained crud product was purified by preparative TLC to obtain compound II[20],[21] [Scheme 1].

Synthesis of ethyl 2-(5-amino-1, 3, 4-thiadiazole-2-yl-thio) acetate (III)

Ethyl chloroacetate (6 mmol) was added to a stirred solution of 5-amino-1, 3, 4-thiadiazole-2-thiol (5 mmol) and potassium hydroxide (5 mmol) in (20 mL) absolute ethanol. The reaction mixture was refluxed for 2h, after completion of the reaction, solvent was evaporated in reduced pressure. Extraction was carried out with chloroform and water. Chloroform was evaporated and the remained solid was crystallized with ethanol and collected as white crystal [Scheme 1].[20],[21]

Synthesis of 2-(5-amino-1, 3, 4-thiadiazole-2-ylthio) acetohydrazide (IV)

A mixture of compound III (3 mmol) and hydrazine hydrate (4 mmol) in ethanol (20 mL) was refluxed for 3h. The obtained precipitate was filtered, washed with cold water, dried, and recrystallized from ethanol to give compound IV [Scheme 1].[14],[22],[23],[24]

Synthesis of N’-(4-nitrobenzylidene)-2-(5-amino-1, 3, 4-thiadiazole-2-ylthio) acetohydrazide (V)

Compound IV (2 mmol) was dissolved in 20 mL of absolute methanol then, 4-nitro-benzaldehyde (2 mmol), and glacial acetic acid (3 drops) were added and refluxed for 2h in 20°C. The reaction mixture was cooled and obtained precipitate, filtered, and crystallized from methanol [Scheme 1].[23],[24]

Synthesis of 5-(4-nitrophenyl)-1, 3, 4-thiadiazole-2-amine (VI)

A mixture of 4-nitrobenzoic acid (3 mmol), thiosemicarbazide (3 mmol) and phosphorus oxychloride (20 mL) was refluxed gently for 3h. The mixture was cooled to room temperature, then water was added (90 mL) and the suspension was refluxed for 2h another followed by filtration. The solution was neutralized with sodium hydroxide. The new formed precipitate was filtered and crystallized from ethanol [Scheme 2].[21],[25],[26]
Scheme 2: Synthesis of the target compound VII. POCl3, 3h reflux; ClCOCH2Cl, DCM, Et3N, 1h stirred at room temperature

Click here to view

Synthesis of 2-chloro-N-(5-(4-nitrophenyl)-1, 3, 4-thiadiazole-2-yl) acetamide (VII)

To a solution of 5-(4-nitrophenyl)-1, 3, 4-thiadiazole-2-amine (VI) (2 mmol) and triethylamine (2 mmol) in dichloromethane (20 mL) was added chloroacethylchloride (4 mmol). The reaction mixture was stirred at room temperature for 1h. The resulting solid was filtered and washed with water, and then was crystallized in methanol to give compound (VII) [Scheme 2].[27]

Biological assays

The stock solutions of compounds (1mM and 1 mL) were prepared in dimethylsulfoxide (DMSO) and appropriately diluted with the Roswell Park Memorial Institute (RPMI) for amastigotes and phosphate-buffered saline (PBS) for promastigote to obtain 25, 50, 100, and 200 µM concentrations.

Antileishmanial activity against leishmania promastigote

The promastigote form of L. major (vaccine strain MRHO/IR/75/ER) obtained from the Department of Parasitological, Isfahan University of Medical Sciences, Iran was grown first in Novy–MacNeal–Nicolle and then in RPMI-1640 to produce a mass of this parasite. RPMI-1640 medium was enriched with 10% fetal bovine serum (FBS), penicillin (100 IU/mL), and streptomycin (100 µg/mL). The growth of promastigotes was monitored daily using microscope. Cultures were passaged during 4-day incubation at 37°C and 5% CO2.[3],[5]

100 µL of medium containing 1.125 × 106 parasites in stationary phase was poured in each well of 96-well plates. Then 100 µL of synthesized compounds at 25, 50, 100, and 200 µM concentrations were added to determine the effect on promastigote. Glucantime with the same concentrations as final compounds was used as the positive control. A well devoid of the studied compounds as a negative control was included in the study. Plates incubated at 24°C and evaluated after 24, 48, and 72h. Afterward, neobar lamella were used for observing results and counting the number of promastigotes. Three independent tests in triplicate were done for each compound for 24, 48, and 72h.[3],[5]

Antileishmanial activity against Leishmania amastigotes

J774 mouse macrophage cell line was cultured in RPMI supplemented with 10% FBS, penicillin (100 IU/mL), and streptomycin (100 µg/mL) at 37°C. Upon reaching 70% of confluence, cells were passaged.[3]

At first, 24 × 24 sterile glass cover slips were set on the bottom of each well, then 700 µL of J774 macrophage cells (5× 105 cells/mL) were seeded in 6-well plates and the plates were incubated at 37°C and 5% CO2 for 24–48h to stick macrophages to the bottom of the plate and glass cover slips.[3] Then macrophages were infected with metacyclic L. major promastigotes at a parasite/macrophage ratio of 10:1 and incubated at 37°C in a humidified incubator with 5% CO2 for 24h.

After removal of extracellular parasites by washing with PBS, 1 mL of different concentrations of compounds 25, 50, 100, and 200 µM were added. Glucantime with the concentrations 25, 50, 100, and 200 µM was also added to the container as the positive control. Three sets of experiments were carried out for each compound at 24, 48, and 72h. Afterward cover slips were fixed with methanol, stained with 10% giemsa, and the results were observed using oil-immersion light microscopy. The average number of parasites per macrophage were determined in 100 macrophages [Figure 1].[3]

  Results Top


2-(5-Amino-1, 3, 4-thiadiazol)-1-(4-bromophenyl) ethanone (I)

Yield: 81%, white solid, m.p.88–89°C (lit: 88–91°C),[28] IR νmax, 3330, 3122 (NH2), 2950 (C–H), 1678 (C=O), and 1611 (C=N) cm–1.

2-(5-(4-Nitrobenzylideneamino)-1, 3, 4-thiadiazol-2-ylthio)-1-(4-bromophenyl) ethanone(II)

Yield: 69.44%, yellow solid, m.p.99–101°C, IR νmax, 2920 (C–H), 1711 (C=O), 1680 (C=N), 1344, 1528 (NO2), 1HNMR: (400 MHz; CDCl3): δ 8.74 (1H, s, CH=N), 8.36 (2H, d, J = 8 Hz, Ha), 8.07 (2H, d, J = 8 Hz, Hb), 7.72–7.74 (2H, m, Hc), 7.54–7.57 (2H, m, Hd), 4.25 (1H, d, J = 8 Hz, CH2), and 4.23 (1H, d, J = 8Hz, CH2).

Ethyl 2-(5-amino-1, 3, 4-thiadiazole-2-yl-thio) acetate (III)

Yield: 57%, white solid, m.p.82–83°C (lit: 83–84°C),[8] IR νmax, 3396, 3280 (NH2), 2923 (C–H), 1735 (C = O), and 1300 (C–O) cm–1.

2-(5-Amino-1, 3, 4-thiadiazole-2-ylthio) acetohydrazide (IV)

Yield: 53.78%, white solid, m.p.150–151°C, IR νmax, 3300, 3274, 3029, 1555 (NH2, NH), 1685 (C=N), 1648 (C=O) cm–1, 1HNMR: (400 MHz; DMSO; d6):9.28 (1H, s, NHCO), 7.32 (2H, s, NH2), 4.31(2H, s, CH2), 3.71(2H, s, NH2), MS (m/z): 205(M+), and MW 205g/mol.

N’-(4-Nitrobenzylidene)-2-(5-amino-1, 3, 4-thiadiazole-2-ylthio) acetohydrazide (V)

Yield: 49.31%, yellow solid, m.p.123–125°C, IR νmax, 3441, 3189 (NH2, NH), 1678 (C=N), 1640 (C=O), 1580, 1342 (NO2) cm–1, 1HNMR: (400 MHz; DMSO; d6): 11.94 (1H, s, CH=N), 8.28 (2H, d, J=8HZ, Ha), 8.1 (1H, s, NH), 7.93–7.99 (2H, d, J=8Hz, Hb), 7.3 (2H, s, NH2), 4.35 (2H, s, CH2), MS (m/z): 338 (M+), and MW 338g/mol.

5-(4-Nitrophenyl)-1, 3,4-thiadiazole-2-amine (VI)

Yield: 91%, yellow solid, m.p. 256–258°C (lit: 258–260 °C),[26] IR νmax, 3441, 3189(NH2), 3090(C–H), 1599, and 1336 (NO2) cm–1.

2-Chloro-N-(5-(4-nitrophenyl)-1, 3, 4-thiadiazole-2-yl) acetamide(VII)

Yield: 88%, pale white, m.p.149–150°C, IR νmax, 3100(NH), 2936(C–H), 1573, 1342(NO2) cm–1, 1HNMR: (400 MHz; CDCl3):8.39 (2H, d, J=8Hz, Ha), 8.18(2H, d, J=8Hz, Hb), 8.12(1H, s, NH), 4.42(2H, s, CH2), MS (m/z): 298 (M+), and MW 298.5g/mol.

Antileishmanial activity

The half maximal inhibitory concentration (IC50) values (µM) of compounds against promastigotes and amastigote after 24-, 48-, and 72-h incubation are presented in [Tables 1] and [2]. The lowest IC50 values against promastigotes belonged to the compounds II (in 24-, 48-, and 72-h incubation) and IV, V and VII (in 72-h incubation). The most potent compounds against amastigote were found to be II (in 24-, 48-, and 72-h incubation), VII (48h) and V, VI, VII (72h). The average number of amastigotes in 100 macrophages in three different treatments with final compounds and with glucantime and without any antileishmanial agent is shown graphically in [Figure 2]. Analysis of variance (ANOVA) analysis showed that the antiamastigotes effects of all compounds at all concentrations were significant in comparison with the negative control (P <0.001), as shown in [Figure 2]. The IC50 values of the test derivatives indicated that all derivatives showed better activity than glucantime as reference drug against promastigote after 72-h incubation.
Table 1: Half maximal inhibitory concentration (IC50) values (µM) for antileishmanial activity against promastigote of the studied compounds

Click here to view
Table 2: Half maximal inhibitory concentration (IC50) values (µM) for antileishmanial activity against amastigote of the studied compounds

Click here to view
Figure 2: In vitro activity of target compounds against intramacrophage amastigotes of Leishmania major. (A) The mean number of amastigotes per 100 macrophages after treatment with target compounds for 24h. (B) The mean number of amastigotes per 100 macrophages after treatment with target compounds for 48h. (C) The mean number of amastigotes per 100 macrophages after treatment with the target compounds for 72h. (*P < 0.001 vs. negative control; ◼ P < 0.001 vs. Glu-25, compounds-25; ◆ P < 0.001 vs. Glu-50, compounds-50; ▲P < 0.001 vs. Glu-100, compounds-100; ● P < 0.001 vs. Glu-200, compounds-200

Click here to view

  Discussion Top

Many of 1, 3, 4-thiadiazoles derivatives were synthesized and evaluated in the past years in antileishmanial experiments.[29],[30],[31] In this study, a series of 2-substituted-thio-1, 3, 4-thiadiazoles bearing (4-nitrobenzylideneamino) or amine at the 5-position (II, IV, V) and one derivative of 2-substituted-amino-1, 3, 4-thiadiazole bearing (4-nitrophenyl) (VII) at the 5-position were synthesized and evaluated against promastigote and amastigote forms of L. major.

The resulting data revealed that all of the synthesized compounds showed good antleishmanial activity against both forms of L. major after 48- and 72-h incubation. IC50 values against promastigote forms were <60 and <10 µM after 48- and 72-h incubation, respectively. Also, these compounds showed antiamastigote activity with IC50 values <50 and <20 µM after 48- and 72-h incubation, respectively.

According to SAR studies, existence various nitroaryl derivatives at C-5 and large groups connected to the 2-position of thiadiazole ring were liable for the antileishmania effects.[16] Previous studies illustrated that an amine substituent at C-2 position of 5-(nitroheterocycle -2-yl)-1, 3, 4-thiadiazoles has profound effect in the antileishmanial activity of these compounds.[1],[5]

The comparison of IC50 values for compounds IV and V showed nearly similar effect against both forms of the parasite. Literature surveys have shown that that the C-2 substituent in 5-(nitroheteroaryl)-1, 3, 4-thiadiazoles is commutable to determine the potency and physicochemical properties of these derivatives. The substitutions of the linear in the C-2 position of 1, 3, 4-thiadiazole ring increased the in vitro activity against promastigotes, whereas the link of cyclic group showed lower antipromastigote activity due to a steric hindrance around the C-2 position.[4]

The comparison of IC50 values for compounds II and IV, V showed that the presence of bulky the substitution at 5 position could improve antileishmania activity as seen in the case of compound II. By comparing the IC50 values of 2-substituted-amino-1, 3, 4-thiadiazole VII with 2-substituted-thio-1, 3, 4-thiadiazole II, it was revealed that halogen substituent slightly increased the antipromastigote activity after 72-h incubation.

  Conclusion Top

Compound II was the most potent compound after 24-, 48- and 72-h incubation against both forms of the parasite, whereas compounds IV, V, and VII indicated the same result after the same periods of incubation time against promastigote and amastigote forms. In addition, compound II was more active than the reference drug after 24-, 48-, and 72-h incubation against both forms of the parasite.

Financial support and sponsorship

The content of this paper was obtained from the PharmD thesis, which was financially supported by the Research Council of Isfahan University of Medical Sciences, Isfahan, I.R. Iran (Grant No. 397308).

Conflicts of interest


  References Top

Tahghighi A, Razmi S, Mahdavi M, Foroumadi P, Ardestani SK, Emami S, et al. Synthesis and antileishmanial activity of 5-(5-nitrofuran-2-yl)-1,3,4-thiadiazol-2-amines containing N-[(1-benzyl-1h-1,2,3-triazol-4-yl)methyl] moieties. Eur J Med Chem 2012;50:124-8.  Back to cited text no. 1
Tahghighi A, Marznaki FR, Kobarfard F, Dastmalchi S, Mojarrad JS, Razmi S, et al. Synthesis and antileishmanial activity of novel 5-(5-nitrofuran-2-y1)-1,3,4-thiadiazoles with piperazinyl-linked benzamidine substituents. Eur J Med Chem 2011;46:2602-8.  Back to cited text no. 2
Sheikhmoradi V, Saberi S, Saghaei L, Pestehchian N, Fassihi A Synthesis and antileishmanial activity of antimony (V) complexes of hydroxypyranone and hydroxypyridinone ligands. Res Pharm Sci 2018;13:111-20.  Back to cited text no. 3
Tahghighi A, Foroumadi A, Ardestani SK,Mahdian SMA Synthesis and comparison of in vitro leishmanicidal activity of 5-nitroheteroaryl)-1,3, 4-thiadiazols containing cyclic amine of piperidin-4-ol at C-2 with acyclic amine analogues against Iranian strain of Leishmania major (MRHO/IR/75/ER). J Arthropod-Borne Dis 2017;11:95-104.  Back to cited text no. 4
Tahghighi A, Emami S, Razmi S, Rezazade Marznaki F, Kabudanian Ardestani S, Dastmalchi S, et al. New 5-(nitroheteroaryl)-1,3,4-thiadiazols containing acyclic amines at C-2: Synthesis and SAR study for their antileishmanial activity. J Enzyme Inhib Med Chem 2013;28:843-52  Back to cited text no. 5
Tahghighi A, Babalouei F Thiadiazoles: The appropriate pharmacological scaffolds with leishmanicidal and antimalarial activities: A review. Iran J Basic Med Sci 2017;20:613-22.  Back to cited text no. 6
Pathak D, Yadav M, Siddiqui N, Kushawah S Antileishmanial agents: An updated review. Der Pharma Chem 2011;3:239-49.  Back to cited text no. 7
Rad O, TiborI R, Duma M, Vlase L, Pirnau A, Tiperciuc B, et al. Synthesis and antimicrobial activity of some 5-amino-2-mercapto-1,3,4-thiadiazole derivatives Thioeters and Schiff bases. Studia Ubb Chemia 2016;61:17-32.  Back to cited text no. 8
Jain AK, Sharma S, Vaidya A, Ravichandran V, Agrawal RK 1,3,4-thiadiazole and its derivatives: A review on recent progress in biological activities. Chem Biol Drug Des 2013;81:557-76.  Back to cited text no. 9
Serban G Future prospects in the treatment of parasitic diseases: 2-amino-1,3,4-thiadiazoles in leishmaniasis. Molecules 2019;24:1-22.  Back to cited text no. 10
Aliabadi A, Eghbalian E, Kiani A Synthesis and evaluation of the cytotoxicity of a series of 1,3,4-thiadiazole based compounds as anticancer agents. Iran J Basic Med Sci 2013;16:1133-8.  Back to cited text no. 11
Jalhan S, Jindal A, Gupta A Synthesis, biological activities and chemistry of thiadiazole derivatives and Schiff bases. Asian J Pharm Clin Res 2012;5:199-208.  Back to cited text no. 12
Asif M A mini review on pharmacological activities of oxadiazole and thiadiazole compounds. Mor J Chem 2014;2:70-84.  Back to cited text no. 13
Rezki N, Al-Yahyawi AM, Bardaweel SK, Al-Blewi FF,Aouad MR Synthesis of novel 2,5-disubstituted-1,3,4-thiadiazoles clubbed1,2,4-triazole, 1,3,4-thiadiazole, 1,3,4-oxadiazole and/or Schiff base as potential antimicrobial and antiproliferative agents. Molecules 2015;20:16049-067.  Back to cited text no. 14
Foroumadi A, Pournourmohammadi S, Soltani F, Asgharian-Rezaee M, Dabiri S, Kharazmi A, et al. Synthesis and in vitro leishmanicidal activity of 2-(5-nitro-2-furyl) and 2-(5-nitro-2-thienyl)-5-substituted-1,3,4-thiadiazoles. Bioorg Med Chem Lett 2005;15:1983-5.  Back to cited text no. 15
Alipour E, Emami S, Yahya-Meymandi A, Nakhjiri M, Johari F, Ardestani SK, et al. Synthesis and antileishmanial activity of 5-(5-nitroaryl)-2-substituted-thio-1,3,4-thiadiazoles. J Enzyme Inhib Med Chem 2011;26:123-8.  Back to cited text no. 16
Foroumadi A, Adibi H, Ardestani SK, Shirooie S, Bozorgomid A, Jafari A Synthesis and Leishmanicidal Activity of 1-[5-(5-Nitrofuran-2-yl)-1, 3,4-Thiadiazole-2-yl]-4-Benzoyle Piperazines. Iranian J Pharm Res 2017;16:904-9.  Back to cited text no. 17
Al-Qahtani A, Siddiqui YM, Bekhit AA, El-Sayed OA, Aboul-Enein HY, Al-Ahdal MN Inhibition of growth of Leishmania donovani promastigotes by newly synthesized 1,3,4-thiadiazole analogs. Saudi Pharm J 2009;17:227-32.  Back to cited text no. 18
Ram VJ, Goel A, Kandpal M, Mittal N, Goyal N, Tekwani BL, et al. Tetraazanaphthene, tetraazaphenalene and 1,3,4-thiadiazole derivatives as potential leishmanicides. Biorg Med Chem Lett 1997;7:651-56.  Back to cited text no. 19
Alwan SM Synthesis and preliminary antimicrobial activities of new arylideneamino-1,3,4-thiadiazole-(thio/dithio)-acetamido cephalosporanic acids. Molecules 2012;17:1025-38.  Back to cited text no. 20
Baghel US, Kaur H, Chawla A, Dhawan RK Synthesis and antimicrobial evaluation of thiadiazole derivatives. Der Pharma Chem 2014;6:66-9.  Back to cited text no. 21
Al-Azzawi AM, Hamd AS Synthesis, characterization and antimicrobial 1activity evaluation of new cyclic imides containing 1,3,4-thiadiazole and 1,3,4-oxadiazole moieties. Int J Res Pharm Chem 2013;3:890-97.  Back to cited text no. 22
Rehman AU, Rasool S, Abbasi MA, Siddiqui SZ, Sattar A, Khan KM, et al. Synthesis, characterization and biological activity of some new s-substituted derivatives of 5-(2-chlorophenyl)-1,3,4-oxadiazol-2-thiol. J Chem Soc Pak 2015;37:776-86.  Back to cited text no. 23
Kumar CTK, Keshavayya J, Rajesh TN, Peethambar SK, Shoukat AAR Synthesis, characterization, and biological activity of 5-phenyl-1,3,4-thiadiazole-2-amine incorporated azo dye derivatives. Org Chem Int2013;2013:1-7.  Back to cited text no. 24
Zhang F, Wang XL, Shi J, Wang SF, Yin Y, Yang YS, et al. Synthesis, molecular modeling and biological evaluation of N-benzylidene-2-((5-(pyridin-4-yl)-1,3,4-oxadiazol-2-yl)thio)acetohydrazide derivatives as potential anticancer agents. Bioorg Med Chem 2014;22:468-77.  Back to cited text no. 25
Foroumadi A, Daneshtalab M, Shafiee A Synthesis and in vitro Antifungal Activity of 2-Aryl-5-phenylsulfonyl-1,3,4-thiadiazole Derivatives. Arzneim Forsch 1999;49:1035-8.  Back to cited text no. 26
Cressier D, Prouillac C, Hernandez P, Amourette C, Diserbo M, Lion C, et al. Synthesis, antioxidant properties and radioprotective effects of new benzothiazoles and thiadiazoles. Bioorg Med Chem 2009;17:5275-84.  Back to cited text no. 27
Agrawal KM, Talele GS Synthesis and antibacterial, antimycobacterial and docking studies of novel N-piperazinyl fluoroquinolones. Med Chem Res 2013;22:818-31.  Back to cited text no. 28
Vosooghi M, Sabourian R, Tahghighi A, Mahdavi M, Emami S, Razmi S Synthesis, antileishmanial activity and QSAR study of (1,3,4thiadiazol-2-ylthio) acetamides derived from 5-nitrofuran. Med Chem Res 2015;24:891-900.  Back to cited text no. 29
Pourrajab F, Forouzannia SK, Tabatabaee SA Novel immunomodulatory function of 1,3,4-thiadiazole derivatives with leishmanicidal activity. J Antimicrob Chemother 2012;67: 1968-78.  Back to cited text no. 30
Poorrajab F, Ardestani SK, Foroumadi A, Emami S, Kariminia A, Behrouzi-Fardmoghadam M, et al. Selective leishmanicidal effect of 1,3,4-thiadiazole derivatives and possible mechanism of action against Leishmania species. Exp Parasitol 2009;121:323-30.  Back to cited text no. 31


  [Figure 1], [Figure 3], [Figure 4], [Figure 2]

  [Table 1], [Table 2]

This article has been cited by
1 Synthesis, Cytotoxic Effect Assessment and Molecular Docking Studies of Disubstituted Thiadiazole Including Oxadiazole as Hybrid Component
Hacettepe University Journal of the Faculty of Pharmacy. 2022;
[Pubmed] | [DOI]


Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

  In this article
Materials and Me...
Article Figures
Article Tables

 Article Access Statistics
    PDF Downloaded403    
    Comments [Add]    
    Cited by others 1    

Recommend this journal