|Year : 2021 | Volume
| Issue : 2 | Page : 209-215
Effects of Aegle marmelos (L.) methanolic leaf extracts on biochemical parameters in diabetic rats
Ravi Babu Birudu1, Padmavathi Pamulapati1, Sathish Kumar Manoharan2
1 Department of Zoology & Aquaculture, Acharya Nagarjuna University, Guntur, India
2 Department of Pharmacology, Bapatla College of Pharmacy, Bapatla, Andhra Pradesh, India
|Date of Submission||21-Oct-2020|
|Date of Acceptance||16-Oct-2021|
|Date of Web Publication||17-Dec-2021|
Dr. Padmavathi Pamulapati
Department of Zoology & Aquaculture, Acharya Nagarjuna University, Guntur 522510, Andhra Pradesh.
Source of Support: None, Conflict of Interest: None
Background: Aegle marmelos (L.) Correa is a widely found plant in India as well as in South Asia. For more than several centuries, it is being widely used for its medicinal properties. Objective: The objective of this study was to evaluate the biochemical changes in alloxan-induced diabetic rats treated with methanolic leaf extracts of A. marmelos. Materials and Methods: Six treatment groups (namely control, diseased, standard (glimepiride), low dose (100 mg/kg), medium dose (250 mg/kg), and high dose (500 mg/kg) of methanolic leaf extracts were used in the study. The biochemical effects were evaluated by the determination of albumin-to-globulin ratio (A/G ratio), albumin, amylase, bilirubin, blood urea, blood urea nitrogen, calcium, direct bilirubin, globulin, glucose-6-phosphate, glycated hemoglobin (HbA1c), homocysteine, indirect bilirubin, inorganic phosphate, lipase, mean blood glucose, serum uric acid, and vitamin D3. Results: No significant changes were observed in A/G ratio among the treatment groups when compared with the diseased and control treatment groups. Low- and medium-dose-treated animals showed a significant change in albumin, bilirubin, calcium, direct bilirubin, indirect bilirubin, globulin, glucose-6-phosphate, homocysteine, inorganic phosphate, lipase, and vitamin D3 levels when compared with standard treatment group as well as diseased group. Low-dose treatment group animals showed a significant increase in amylase and mean blood glucose levels than the diseased treatment groups, whereas low-dose treatment group animals showed a significant decrease in HbA1c levels than the diseased treatment groups. Conclusion: Through the biochemical changes, it is evident that the low and medium dose of methanolic leaf extract of A. marmelos can be used in the treatment of diabetes and its complications.
Keywords: Aegle marmelos, alloxan, bael, diabetes, Rutaceae
|How to cite this article:|
Birudu RB, Pamulapati P, Manoharan SK. Effects of Aegle marmelos (L.) methanolic leaf extracts on biochemical parameters in diabetic rats. J Rep Pharma Sci 2021;10:209-15
|How to cite this URL:|
Birudu RB, Pamulapati P, Manoharan SK. Effects of Aegle marmelos (L.) methanolic leaf extracts on biochemical parameters in diabetic rats. J Rep Pharma Sci [serial online] 2021 [cited 2022 Jan 17];10:209-15. Available from: https://www.jrpsjournal.com/text.asp?2021/10/2/209/332770
| Introduction|| |
Natural products have a very special place in drug research and development. Plants as a source of therapeutically useful drugs have been proved to the evidence of high economic importance. Search for new drugs from various plant sources occurs throughout the globe. In India though there are certain limitations or challenges in the resources, standardization of medicinal plants has gained significance in recent times.
Aegle marmelos (L.) Correa is a widely found medicinal plant in India and South Asia. It is being commonly used for its therapeutic properties. Analgesic, antioxidant, antibacterial, antifungal, anticancer, antidiarrheal, immunomodulant, antihyperlipidemic, antiulcer, diuretic, antifilarial, and hepatoprotective activities have been reported in various plant extracts of the plant.,,,,,,,,,,,,,,,,,,,,,,,,, Most of the phytocompounds are found to be accumulated in the leaves of the plants. Therefore, the present research work aimed at the evaluation of biochemical changes in diabetic rats treated with methanolic leaf extracts from A. marmelos.
| Materials and Methods|| |
Collection of plant material
The leaves of A. marmelos (L.) were collected from Dolas Nagar, Tadepalli Mandal, Guntur District, Andhra Pradesh, India. Authentication was performed by Dr. P. Satya Narayana Raju, Plant Taxonomist, Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India. The reference specimen is preserved in the Department of Botany, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur.
Preparation of plant extracts
The collected leaves were washed thoroughly with water and shade dried. Methanolic leaf extracts were obtained by extracting powder with 85% ethanol by Soxhlet extraction method for 72 h. After completion of the extraction, the excess solvent was removed by rotary evaporation. The methanolic leaf extract was used for further evaluation of biochemical changes in alloxan-induced diabetes.
Preliminary phytochemical analysis
The methanolic leaf extract from A. marmelos (L.) was subjected to preliminary phytochemical analysis to assess the presence of various phytoconstituents; it revealed the presence of glycosides, saponins, tannins, and flavonoids.
Normal healthy male Wistar albino rats, 9–12 weeks old with an average weight of 200–250 g, were procured from the Mahaveer Enterprises (CPCSEA Regd No: 146/99/CPCSEA), Bagh Amberpet, Hyderabad. They were housed in polypropylene cages and fed with a standard chow diet and water ad libitum.
The animals were acclimatized to the conditions by maintaining them at a temperature 25 ± 2°C and relative humidity 55 ± 10 at 12 h each at dark and light cycle for about 7 days prior to dosing and during the commencement of the experiment.
All experimental procedures involving animals were conducted in accordance with the guidelines of the Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA) with prior approval from the Institutional Animal Ethics Committee (IAEC Approval No. ANUCPS/IAEC/AM/P/26/2019) of College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India.
The biochemical changes were evaluated using alloxan-induced diabetes model. A total of 36 rats were used. The rats were divided into six groups of six rats each. Group 1: Vehicle treatment group; Group 2: Disease control; Group 3: Standard treatment (glimepiride 40 mg/kg); Group 4: Low dose of methanolic leaf extract (100 mg/kg); Group 5: Medium dose of methanolic leaf extract (250 mg/kg); and Group 6: High dose (500 mg/kg). Plant leaf extracts were suspended in a vehicle solution of 0.5% dimethyl sulfoxide [DMSO] and a dose of l mL/kg; body weight was administered orally using an intragastric tube for 15–45 days to the respective groups.
Alloxan monohydrate was procured from Sigma Aldrich, Bangalore, India. All the other chemicals and solvents used in the study were of analytical grade and obtained from local suppliers.
Acute toxicity studies
The acute toxicity studies were carried out in accordance with OECD Test Guideline 423: Acute Oral Toxicity––Acute Toxic Class Method. The methanolic leaf extract of A. marmelos (L.) was found to be safe up to 2000 mg/kg body weight after oral administration of the test compound. 100 mg/kg, 250 mg/kg, and 500 mg/kg were used for further animal pharmacological study.
Diabetes was induced by the administration of alloxan monohydrate (150 mg/kg b.w.,) with normal saline as vehicle. After 72 h, rats with blood glucose levels more than 150 mg/dL were selected for further biochemical evaluation. The blood glucose levels were estimated using one-touch glucometer. The biochemical effects were evaluated by the determination of albumin-to-globulin ratio (A/G ratio), albumin, amylase, bilirubin, blood urea, blood urea nitrogen, calcium, direct bilirubin, globulin, glucose-6-phosphate, HB1Ac, homocysteine, indirect bilirubin, inorganic phosphate, lipase, mean blood glucose, serum uric acid, and vitamin D3 [Figure 1].
|Figure 1: Graphical summary of evaluation of effects of plant extracts on diabetic rats|
Click here to view
Results of the study were presented as mean ± standard error of the mean. The statistical significance of the groups was determined using one-way analysis of variance followed by Dunnett’s test using Graph Pad PRISM software and a value of P < 0.05 was considered as significant.
| Results and Discussion|| |
Drugs from plants are researched in laboratory animals both for therapeutic efficacy and safety. This is significant on the grounds that any hepatic and renal harm will modify design and capacity of these fundamental organs and effects in general digestion. The liver is the main organ in the metabolism of plant drugs and different substances. Liver cell obliteration shows its belongings generally as significant in the liver cell film penetrability, which brings about the spilling out of tissue content into the circulatory system. In a few organs, cell film harm is trailed by arrival of various cytoplasmic chemicals to the blood, a marvel that gives the premise to clinical analysis. Abnormal biochemical changes in bilirubin, direct bilirubin, indirect bilirubin, D3, amylase, blood urea, blood urea nitrogen, calcium, globulin, glucose-6-phosphate, homocysteine, inorganic phosphate, lipase, serum uric acid, and vitamin levels are of clinical and toxicological significance, being characteristic of tissue harm by poisons or infection condition.
Effects of methanolic leaf extracts on serum albumin-to-globulin ratio of the treated animals
Higher A/G ratio is an indication of disease in the liver, kidney, or intestines. It is also linked to diabetes, low thyroid activity, and leukemia. Similar to earlier studies, when compared to normal control rats, alloxan-induced diabetic rats had significantly higher serum A/G ratio concentrations. No significant changes were observed in serum A/G ratio among the treatment groups when compared with the diseased (1.81 ± 0.81) and control treatment groups (1.74 ± 0.10). The statistical significance between the groups was found to be P > 0.05. The effects of methanolic leaf extracts on serum A/G ratio of the treated rats are shown in [Table 1].
|Table 1: Effects of Aegle marmelos (L.) methanolic leaf extracts on albumin-to-globulin ratio in diabetic rats|
Click here to view
Effects of methanolic leaf extracts on bilirubin, direct bilirubin, and indirect bilirubin levels of the animals
In the systemic circulation, bilirubin is the end result of hemoglobin breakdown. Stocker et al. discovered in 1987 that bilirubin served as a physiological antioxidant. Recent research has discovered that a slight increase in bilirubin levels within the physiological range protects against metabolic disorders. The insulin resistance status may have a role in the connection between certain metabolic disorders and bilirubin.
Bilirubin may play a role in the insulin signaling pathway’s signal transduction, improving insulin sensitivity by lowering oxidative stress and inflammatory reactions. The physiological properties of bilirubin may explain its ability to protect against the progression of diabetes. These properties make bilirubin a potential clinical biomarker or therapeutic target for a variety of disease states. Similar to earlier studies, an extremely significant increase in the bilirubin, direct bilirubin, and indirect bilirubin levels was observed in the standard, low-, and medium-dose-treated animals (142.3 ± 20.52) when compared to the normal group animals. The statistical significance between the groups was found to be P < 0.05. The effects of methanolic leaf extracts on bilirubin, direct bilirubin, and indirect bilirubin levels of the treated rats are shown in [Table 2].
|Table 2: Effects of methanolic leaf extracts on bilirubin, direct bilirubin, and indirect bilirubin levels in diabetic rats|
Click here to view
Effects of methanolic leaf extracts on albumin levels of the animals
Blood plasma proteins are the first to get modified as they are directly exposed to higher glucose concentrations and a number of them have been identified. Human serum albumin is one of the most abundant plasma proteins and is heavily glycated in diabetes. Albumin constitutes more than 50% of plasma proteins, and any variation in levels of albumin may change the stoichiometry of glycation of other plasma proteins glycation. In diabetic patients, albuminuria is an important predictive marker for progressive diabetic renal impairment, cardiovascular disease, and diabetic retinopathy. Untreated diabetic animals developed albuminuria, which could be caused by decreased tubular reabsorption or albumin leakage due to a damaged glomerular membrane. Similar to the earlier studies, an extremely significant increase in albumin levels was observed in the standard, low-, and medium-dose-treated animals when compared to the normal group animals. The statistical significance between the groups was found to be P < 0.05. The effects of methanolic leaf extracts on the albumin levels of the treated rats are shown in [Table 3].
|Table 3: Effects of methanolic leaf extracts on albumin levels in diabetic rats|
Click here to view
Effects of methanolic leaf extracts on amylase, blood urea, blood urea nitrogen, calcium, globulin, glucose-6-phosphate, homocysteine, inorganic phosphate, lipase, serum uric acid, and vitamin D3 levels of the animals
Insulin inhibits HMG-CoA reductase, which creates cholesterol, and activates lipoprotein lipase, which hydrolyzes triglycerides, in normal conditions. Hypercholesterolemia and hypertriglyceridemia arise as a result of insulin insufficiency in diabetes. As a result, treatment with a polyherbal formulation may be responsible for lowering lipid levels via insulin release or insulin sensitization.
Phosphorus is important for energy storage, transmission, and liberation in the body, as well as intermediary metabolism of carbs, fats, and proteins. Pi is an inorganic phosphate that is found in DNA and RNA and is involved in both glycolysis and oxidative phosphorylation. Pi is a substrate for glyceraldehyde-3-phosphate dehydrogenase, which accelerates glycolysis. Mitochondria are our bodies’ power factories, and their major job is to produce adenosine triphosphate (ATP), which provides 90%–95% of all cellular energy through oxidative phosphorylation. As has been well documented, the concentration of plasma Pi, and hence red cell 2,3-diphosphoglycerate and ATP levels, and oxygen transport to tissue are intimately linked with diabetes and other diseases (hyperalimentation and uremia).
Similar to earlier studies, an extremely significant increase in the amylase, blood urea, blood urea nitrogen, calcium, globulin, glucose-6-phosphate, homocysteine, inorganic phosphate, lipase, serum uric acid, and vitamin D3 levels was observed in the standard, low-, and medium-dose-treated animals when compared to the normal group animals. The statistical significance between the groups was found to be P < 0.05. The effects of methanolic leaf extracts on amylase, blood urea, blood urea nitrogen, calcium, globulin, glucose-6-phosphate, homocysteine, inorganic phosphate, lipase, serum uric acid, and vitamin D3 levels of the treated rats are shown in [Table 4].
|Table 4: Effects of methanolic leaf extracts on amylase, blood urea, blood urea nitrogen, calcium, globulin, glucose-6-phosphate, homocysteine, inorganic phosphate, lipase, serum uric acid, and vitamin D3 levels in diabetic rats|
Click here to view
Effects of methanolic leaf extracts on glycated hemoglobin levels of the animals
The diabetic untreated rats had considerably higher glycosylated hemoglobin (HbA1c) levels than the normal control rats. This could be due to the action of alloxan, which caused hyperglycemia by causing insulin secretion to decrease as a result of pancreatic beta-cell death. Increased blood glucose causes nonenzymatic adduction of glucose to the free amino groups at the N-terminal of hemoglobin’s beta chain, resulting in glycosylated hemoglobin. Similarly, Hb1Ac levels of the low-dose-treated animals were also significantly decreased (5.85 ± 0.18) when compared to the diseased group animals (6.18 ± 0.30). High HbA1c levels mean high risk of diabetes. The statistical significance between the groups was found to be P< 0.0001, which was considered extremely significant. Variation among column means is significantly greater than expected by chance. The effects of methanolic leaf extracts on HbA1c levels of the treated rats are shown in [Table 5].
|Table 5: Effects of methanolic leaf extracts on HbA1c levels in diabetic rats|
Click here to view
Effects of methanolic leaf extracts on mean blood glucose levels of the animals
Elevated blood glucose induces insulin release from pancreatic cells, which increases peripheral glucose consumption and regulates glucose homeostasis via many processes. In diabetics, this is disrupted, resulting in glucose intolerance. Similar to earlier studies, mean blood glucose levels of the low-dose-treated animals were also decreased (104.83 ± 1.24) when compared to the normal group animals (87.66 ± 2.24). The statistical significance between the groups was found to be P < 0.0001, which was considered extremely significant. The effects of methanolic leaf extracts on mean blood glucose levels of the treated rats are shown in [Table 6].
|Table 6: Effects of methanolic leaf extracts on mean blood glucose levels in diabetic rats|
Click here to view
Earlier studies carried out on various leaf, fruit, and bark extracts from the A. marmelos (L.) also showed significant pharmacological evidence for its therapeutic benefits in various animal models for diabetes and its related complications such as diabetic cataract and diabetic retinopathy.,, Though we were are unable to elucidate the exact mechanism of action without the isolation and characterization of phytoconstituents present in the methanolic leaf extracts, based on the earlier studies the observed antidiabetic activity could be due to the presence of the following compounds such as (a) Rutin: (i) through inhibition of tissue gluconeogenesis, (ii) decrease in carbohydrate absorption from the small intestine, (iii) increase of tissue glucose uptake, (iv) stimulation of insulin secretion from beta cells, (v) prevention of Langerhans islet against degeneration, and (vi) decrease the formation of sorbitol, reactive oxygen species, advanced glycation end-product precursors, and inflammatory cytokines, (b) Aegeline––through activation of Akt and Rab proteins, and (c) Lupeol––through inhibition of α-amylase and α-glucosidase. The findings of the earlier and this study in presented in [Figure 2].
|Figure 2: Schematic illustration of the antidiabetic activity of Aegle marmelos methanol leaf extract|
Click here to view
| Conclusion|| |
Through the biochemical changes, it is evident that the low dose of methanolic leaf extracts from A. marmelos can be used the treatment of diabetes and its complications. The antidiabetic activity could be attributed to the presence of flavonoids in the extracts. However, there is a need for further cellular and molecular pharmacological studies to elucidate the exact mechanisms for its antidiabetic potential.
The authors are thankful to Acharya Nagarjuna University, Nagarjuna Nagar, Andhra Pradesh for providing necessary laboratory conditions.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Buenz EJ, Schnepple DJ, Bauer BA, Elkin PL, Riddle JM, Motley TJ. Techniques: Bioprospecting historical herbal texts by hunting for new leads in old tomes. Trends Pharmacol Sci 2004;25:494-8.
Mujeeb F, Bajpai P, Pathak N. Phytochemical evaluation, antimicrobial activity and determination of bioactive components from leaves of Aegle marmelos
. Biomed Res Int 2014;10;1-12.
Kothari S, Kushwah A, Kothari D. Involvement of opioid and monoaminergic pain pathways in Aegle marmelos
induced analgesia in mice. Indian J Pharmacol 2013;45:371-5.
] [Full text]
Rathee D, Kamboj A, Sidhu S. Augmentation of hepatoprotective potential of Aegle marmelos
in combination with piperine in carbon tetrachloride model in Wistar rats. Chem Cent J 2018;12:94.
Ibrahim NA, Mohammed MMD, Aly HF, Ali SA, Al-Hady DA. Efficiency of the leaves and fruits of Aegle marmelos
methanol extract (L.) Correa and their relative hepatotoxicity induced by CCL4 and identification of their active constituents by using LC/MS/MS. Toxicol Rep 2018;5:1161-8.
Rejiniemon TS, Arasu MV, Duraipandiyan V, Ponmurugan K, Al-Dhabi NA, Arokiyaraj S, et al
. In vitro
antimicrobial, antibiofilm, cytotoxic, antifeedant and larvicidal properties of novel quinone isolated from Aegle marmelos
(Linn.) Correa. Ann Clin Microbiol Antimicrob 2014;13:48.
Bhatti R, Singh J, Saxena AK, Suri N, Ishar MP. Pharmacognostic standardisation and antiproliferative activity of Aegle marmelos
(L.) Correa leaves in various human cancer cell lines. Indian J Pharm Sci 2013;75:628-34.
] [Full text]
Kumar S, Mahaseth RK, Tiwari M, Sehgal R, Rajora P, Mathur R. Interaction of aqueous leaf extract of Aegle marmelos
(L.) Corr. with cholinergic, serotonergic and adrenergic receptors: An ex vivo
study. Indian J Pharmacol 2015;47:109-13.
Balakumar S, Rajan S, Thirunalasundari T, Jeeva S. Antifungal activity of Aegle marmelos
(L.) Correa (Rutaceae
) leaf extract on dermatophytes. Asian Pac J Trop Biomed 2011;1:309-12.
Mujeeb F, Khan AF, Bajpai P, Pathak N. Phytochemical study of Aegle marmelos
: Chromatographic elucidation of polyphenolics and assessment of antioxidant and cytotoxic potential. Pharmacogn Mag. 2017;13:S791.
Rana BK, Singh UP, Taneja V. Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos
. J Ethnopharmacol 1997;57:29-34.
Kaushik P, Kumar S. Data of de novo
assembly of the leaf transcriptome in Aegle marmelos
. Data Brief 2018;19:700-3.
Subramaniam D, Giridharan P, Murmu N, Shankaranarayanan NP, May R, Houchen CW, et al
. Activation of apoptosis by 1-hydroxy-5,7-dimethoxy-2-naphthalene-carboxaldehyde, a novel compound from Aegle marmelos
. Cancer Res 2008;68:8573-81.
Brijesh S, Daswani P, Tetali P, Antia N, Birdi T. Studies on the antidiarrhoeal activity of Aegle marmelos
unripe fruit: Validating its traditional usage. BMC Complement Altern Med 2009;9:47.
Nair J, Velpandian T, Das US, Sharma P, Nag T, Mathur SR, et al
. Molecular and metabolic markers of fructose induced hepatic insulin resistance in developing and adult rats are distinct and Aegle marmelos
is an effective modulator. Sci Rep 2018;8: 15950.
Govinda HV, Asdaq SM. Immunomodulatory potential of methanol extract of Aegle marmelos
in animals. Indian J Pharm Sci 2011;73:235-40.
] [Full text]
Reddy VP, Urooj A. Antioxidant properties and stability of Aegle marmelos
leaves extracts. J Food Sci Technol 2013;50:135-40.
Jayachandran Nair CV, Ahamad S, Khan W, Anjum V, Mathur R. Development and validation of high-performance thin-layer chromatography method for simultaneous determination of polyphenolic compounds in medicinal plants. Pharmacognosy Res 2017;9:67-73.
Chockalingam V, Kadali SS, Gnanasambantham P. Antiproliferative and antioxidant activity of Aegle marmelos
(Linn.) leaves in Dalton’s lymphoma ascites transplanted mice. Indian J Pharmacol 2012;44:225-9.
] [Full text]
Lalremruta V, Prasanna GS. Evaluation of protective effect of Aegle marmelos
corr: In an animal model of chronic fatigue syndrome. Indian J Pharmacol 2012;44:351-6. [Full text]
Asghar N, Mushtaq Z, Arshad MU, Imran M, Ahmad RS, Hussain SM. Phytochemical composition, antilipidemic and antihypercholestrolemic perspectives of bael leaf extracts. Lipids Health Dis 2018;17:68.
Ilavarasan JR, Monideen S, Vijayalakshmi M. Antiulcer activity of Aegle marmelos
Linn. Anc Sci Life 2002;21:256-9.
Vinodhini R. Detoxifying effect of Nelumbo nucifera
and Aegle marmelos
on hematological parameters of common carp (Cyprinus carpio
L.). Interdiscip Toxicol 2010;3:127-31.
Parmar NA, Patel BR, Nariya MB. A comparative experimental study to evaluate mutrala (diuretic) activity of bilva moola and patra (Aegle marmelos
corr.). Ayu 2014;35:344-7.
] [Full text]
Perumal Samy R, Manikandan J, Al Qahtani M. Evaluation of aromatic plants and compounds used to fight multidrug resistant infections. Evid Based Complement Alternat Med 2013;15:1-17.
Sharma RD, Veerpathran AR, Dakshinamoorthy G, Sahare KN, Goswami K, Reddy MV. Possible implication of oxidative stress in anti filarial effect of certain traditionally used medicinal plants in vitro
against brugia malayi microfilariae. Pharmacognosy Res 2010;2:350-4.
Namsa ND, Tangjang S, Arya SC, Rajbonshi B, Samal PK, Mandal M. An inventory of the ethnobotanicals used as anti-diabetic by a rural community of Dhemaji district of Assam, Northeast India. J Ethnopharmacol 2011;138:345-50.
Liaqat I, Riaz N, Saleem QU, Tahir HM, Arshad M, Arshad N. Toxicological evaluation of essential oils from some plants of Rutaceae
family. Evid Based Complement Alternat Med 2018;15:1-17.
Yadav JP, Saini S, Kalia AN, Dangi AS. Hypoglycemic and hypolipidemic activity of ethanolic extract of salvadora oleoides in normal and alloxan-induced diabetic rats. Indian J Pharmacol 2008;40:23-7.
] [Full text]
Murugan P, Pari L. Influence of tetrahydrocurcumin on hepatic and renal functional markers and protein levels in experimental type 2 diabetic rats. Basic Clin Pharmacol Toxicol 2007;101:241-5.
Zhang F, Guan W, Fu Z, Zhou L, Guo W, Ma Y, et al
. Relationship between serum indirect bilirubin level and insulin sensitivity: Results from two independent cohorts of obese patients with impaired glucose regulation and type 2 diabetes mellitus in China. Int J Endocrinol 2020;2020:5681296.
Ch G, Al-Numair KS, Pugalendi KV. Effect of 3-hydroxymethyl xylitol on hepatic and renal functional markers and protein levels in streptozotocin-diabetic rats. Afr J Biochem Res 2009;31;3:198-204.
Oseni OA, Odesanmi OE, Oladele FC. Antioxidative and antidiabetic activities of watermelon (Citrullus lanatus
) juice on oxidative stress in alloxan-induced diabetic male Wistar albino rats. Niger Med J 2015;56:272-7.
] [Full text]
Gupta PP, Haider J, Yadav RP, Pal U. Preclinical evaluation of antidiabetic activity of poly herbal plant extract in streptozotocin induced diabetic rats. J Phytopharm 2016;5:45-9.
Aba PE, Asuzu IU. Glycosylated haemoglobin values of alloxan-induced diabetic rats treated with graded doses of Cussonia arborea
extract. J Appl Anim Res 2018;46:1478-82.
Kotha P, Badri KR, Nagalapuram R, Allagadda R, Chippada AR. Anti-diabetic potential of the leaves of Anisomeles malabarica
in streptozotocin induced diabetic rats. Cell Physiol Biochem 2017;43:1689-702.
Ponnachan PT, Paulose CS, Panikkar KR. Effect of leaf extract of Aegle marmelos
in diabetic rats. Indian J Exp Biol 1993;31:345-7.
Parveen A, Kim J, Oh B, Subedi L, Khan Z, Kim S. Phytochemicals: Target-based therapeutic strategies for diabetic retinopathy. Molecules 2018;23:1519-26.
Santoshkumari KS, Devi KS. Hypoglycemic effect of a few medicinal plants. Anc Sci Life 1990;9:221-3.
Ghorbani A. Mechanisms of antidiabetic effects of flavonoid rutin. Biomed Pharmacother 2017;96:305-12.
Gautam S, Ishrat N, Singh R, Narender T, Srivastava AK. Aegeline from Aegle marmelos
stimulates glucose transport via AKT and RAC1 signaling, and contributes to a cytoskeletal rearrangement through PI3K/RAC1. Eur J Pharmacol 2015;762:419-29.
Gurupriya S, Cathrine L, Ramesh J. In vitro
antidiabetic and antioxidant activities of lupeol isolated from the methanolic extract of Andrographis echioides
leaves. J Pharmacogn Phytochem 2018;7:768-5.
[Figure 1], [Figure 2]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]