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Hepatoprotective effects of Hertia cheirifolia butanolic extract and selenium against CCl4-induced toxicity in rats
Mouna Menakh1, Saber Boutellaa2, Djahida Mahdi3, Amar Zellagui4, Mesbah Lahouel5, Mehmet Ozturk6
1 Department of Nature and Life Sciences, Faculty of Exact Sciences and Nature and Life Sciences, Oum El Bouaghi University, Oum El Bouaghi, Algeria
2 Laboratory of Bio-molecules and Plant Breeding, Department of Nature and Life Sciences, Faculty of Exact Sciences, Oum El Bouaghi University, Oum El Bouaghi, Algeria; Department of Nature and Life Sciences, University Center Abdehafid Boussouf, Mila, Algeria
3 Department of Nature and Life Sciences, Faculty of Exact Sciences and Nature and Life Sciences, Oum El Bouaghi University, Oum El Bouaghi, Algeria; Laboratory of Animal Eco-Physiology, Department of Biology, Faculty of Sciences, Badji Mokhtar University, Annaba, Algeria
4 Laboratory of Bio-molecules and Plant Breeding, Department of Nature and Life Sciences, Faculty of Exact Sciences, Oum El Bouaghi University, Oum El Bouaghi, Algeria
5 Laboratory of Molecular Toxicology, Faculty of Sciences, Jijel University, Jijel, Algeria
6 Department of Chemistry, Faculty of Sciences, Muğla Sıtkı Koçman University, Mugla, Turkey
Correspondence Address:
Dr. Mouna Menakh
Department of Nature and Life Sciences, Faculty of Exact Sciences and Nature and Life Sciences, Oum El Bouaghi University, Oum El Bouaghi 04000.
Algeria
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jrptps.JRPTPS_25_20
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Background: Hertia cheirifolia, a traditional plant endemic to both Tunisia and Algeria, is used for the treatment of various disorders. This study investigates the antioxidant and protective effects of H. cheirifolia butanolic extract (BEHC) alone and combined with selenium (Se) against carbon tetrachloride (CCl4)-induced liver damage in rats. Experimental Procedure: Thirty male Wistar rats were randomly divided into six groups: (1) normal control, (2) hepatotoxic control, (3) positive control received silymarin 100 mg/kg body weight (bw), (4) BEHC (100 mg/kg bw), (5) BEHC (400 mg/kg bw), and (6) BEHC (400 mg/kg bw) + Se (0,3 mg/kg bw) once daily for 14 consecutive days, followed by hepatotoxicity induction with CCl4 in olive oil 0.6 mL/kg bw intraperitoneally. Some biochemical and oxidative stress parameters were investigated. Quantity and quality of phenolics in BEHC were determined by spectrophotometer and high-performance liquid chromatography with diode-array detection (HPLC-DAD) analysis, respectively. Results and Conclusion: BEHC contained high amounts of total phenolics and flavonoids where seven compounds were identified. The pretreatment with BEHC or with BEHC and Se significantly reduced the levels of plasma aminotransferases (alanine aminotransferase [AST] and aspartate aminotransferase [ALT]), alkaline phosphatase, malondialdehyde (MDA), and increasing glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) levels in hepatic tissues. In conclusion, BEHC has a potent natural antioxidant activity that can be used with Se to reduce hepatotoxicity. |
