• Users Online: 330
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
Year : 2022  |  Volume : 11  |  Issue : 1  |  Page : 98-103

Apoptotic and antiproliferative effects of Mimusops elengi leaf extract in Ehrlich ascites carcinoma cells

1 School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan Deemed to be University, Bhubaneswar, Odisha, India
2 Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India

Correspondence Address:
Dr. Goutam Ghosh
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan Deemed to be University, Bhubaneswar, Odisha 751030
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jrptps.JRPTPS_53_21

Rights and Permissions

Context: Mimusops elengi Linn. (Sapotaceae) is commonly known as bakul. Traditionally, the various parts of the plants have been used in the treatment of wound healing, pain, and tumor. Objective: To evaluate the role of Mimusops elengi extract (MEE) on proliferation, apoptosis, and bcl2 gene expression in Ehrlich ascites carcinoma (EAC) cells lines and establish the possible mechanisms linked with anticancer activity. Settings and Design: EAC cells were treated with methanol MEE (20–400 µg/mL) in time intervals of 24, 48, and 72 h. Materials and Methods: The antiproliferative effect of extract was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; deoxyribonucleic acid (DNA) fragmentation study, cell cycle analysis, and Annexin V-fluorescein isothiocyanate (FITC) assay were performed to assess the apoptosis, and lastly, western blotting study was performed to assess the bands intensities using the ImageJ® analysis (a Java-based image processing program system, National Institutes of Health and the Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI). Statistical Analysis Used: The data were analyzed using one-way analysis of variance followed by the Dunnett’s post hoc test. Results: MEE shows significant antiproliferative effect on EAC cell lines. In the DNA fragmentation assay, it shows significant fragmentation of DNA. In the cell cycle analysis and Annexin V-FITC assay, there was arrested in sub-G1 phase and initiation of cell apoptosis. In western blotting study, the extract shows low expression of bcl-2 and overexpression of Bax proteins. Conclusions: From the above result, it concludes that MEE has significant apoptosis-inducing properties.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded61    
    Comments [Add]    

Recommend this journal