Journal of Reports in Pharmaceutical Sciences

RESEARCH ARTICLE
Year
: 2018  |  Volume : 7  |  Issue : 3  |  Page : 344--356

Protective effect of grape seed extract against acrylamide-induced neurotoxicity in Vitro and In Vivo


Leila Mohammadzadeh1, Soghra Mehri2, Hossein Hosseinzadeh3 
1 Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2 Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Pharmacodynamics and Toxicology, School of Pharmacy; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence Address:
Hossein Hosseinzadeh
Department of Pharmacodynamics and Toxicology, School of Pharmacy; Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad
Iran

Acrylamide (ACR) monomer is an effective neurotoxicant, which damages the central and peripheral nervous systems in humans and animals. It also has broad applications in different industries. Grape seed extract (GSE) possesses antioxidant properties. The present study was designed to evaluate the beneficial effects of GSE against ACR-induced neurotoxicity in both in vitro and in vivo. In our in vitro study, the effect of different concentrations of GSE on ACR toxicity (IC50) in PC12 cells was evaluated using MTT assay. Moreover, in another experiment, the effect of GSE on neural toxicity induced by ACR was evaluated in rats. Pretreatment with GSE (10-100 μM) significantly decreased ACR induced cytotoxicity. In Wistar rats, exposure to ACR (50 mg/kg IP for 11days), significantly induced severe gait abnormalities but treatment with GSE (12.5 mg/kg) reduced ACR induced neurotoxicity in animals. After that, rats were sacrificed and malondialdehyde (MDA) as a marker of lipid peroxidation and GSH content were determined in brain tissue. ACR induced lipid peroxidation and increased level of MDA, while reduced GSH level. Treatment with GSE significantly reduced level of MDA and increased GSH content in cerebral cortex (p<0.001). The results suggest that the neuroprotective effect of GSE in this model in part, may be due to its ability to scavenge free radicals and its effective recovery of the antioxidative defense system.


How to cite this article:
Mohammadzadeh L, Mehri S, Hosseinzadeh H. Protective effect of grape seed extract against acrylamide-induced neurotoxicity in Vitro and In Vivo.J Rep Pharma Sci 2018;7:344-356


How to cite this URL:
Mohammadzadeh L, Mehri S, Hosseinzadeh H. Protective effect of grape seed extract against acrylamide-induced neurotoxicity in Vitro and In Vivo. J Rep Pharma Sci [serial online] 2018 [cited 2022 Jun 28 ];7:344-356
Available from: https://www.jrpsjournal.com/article.asp?issn=2322-1232;year=2018;volume=7;issue=3;spage=344;epage=356;aulast=Mohammadzadeh;type=0